Gas chromatography (GC) and mass spectrometry (MS):
A Thermo Trace 1310 GC interphase to a Thermo Scientific TSQ 8000 EVO triple quadrupole MS allows for the separation, and the untargeted and targeted analysis of biomarkers using full scan and Selected Reaction Monitoring (SRM) modes. The high signal over ratio provided by SRM mode ensures a detection limit of ca. 0.5 fg on column, which is ideal for the analysis of samples with low organic content.
A Thermo Finnigan Trace GC Ultra interphase to a PolarisQ ion-trap MS allows for the further characterization of biomarkers via MS-MS experiments. This instrument can also be attached to a Pyromat Curie-Point pyrolyzer (Brechbühler Scientific Analytical Solutions) for the analysis of bulk samples.
A Hewlett Packard 6890 Gas GC with an FID allows for the separation and accurate quantification of organic molecules.
Gas chromatography (GC)–isotope ratio–mass spectrometry (ir-MS):
A Thermo MAT 253 Plus isotope ratio mass spectrometer equipped with universal CNOS and HD collectors, ConfloIV interface, GC IsolinkII, and TraceGC1310 (GC-IRMS), allows for high sensitivity analysis of carbon and hydrogen stable isotopes of organic molecules.
High performance liquid chromatography (HPLC) and mass spectrometry (MS)
A Thermo Scientific Ultimate 3000 HPLC interphase to a Q Exactive Focus Orbitrap-Quadrupole MS via electrospray ionization (ESI) or atmospheric pressure chemical ionization (APCI) allows for high sensitivity and high mass resolution for the targeted and untargeted analysis of polar biomarkers. Targeted analysis of polar lipids can be performed in Selected Ion Monitoring (SIM) and Parallel Reaction Monitoring (PRM) modes with high-resolution accurate-mass detection, and with enhanced signal over noise ratio for the detection of biomarkers present in low abundance. Untargeted analysis of unknown molecules can be performed via Full scan and Data-Independent Acquisition (DIA) modes. The Ultimate 3000 HPLC is also equipped with a DAD-3000 Diode Array Detector and a Fraction Collector for the identification, quantification, isolation and concentration of organic molecules for further chemical and/or stable isotope analysis.
Other laboratory instrumentation:
Dionex 200 Accelerated Solvent Extraction (ASE) system
Anton Paar Multiwave 3000 Microwave Extraction System
Gilson GX-274 ASPEC Solid Phase Extraction system
Thermo Scientific Thermolyne Furnace Oven
Thermo Scientific Refrigerated Centrifuge
Refrigerator and freezers
Microbalance and analytical balance